Single-cell protein profiling in microchambers with barcoded beads
نویسندگان
چکیده
منابع مشابه
Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies
Human cells express thousands of different surface proteins that can be used for cell classification, or to distinguish healthy and disease conditions. A method capable of profiling a substantial fraction of the surface proteome simultaneously and inexpensively would enable more accurate and complete classification of cell states. We present a highly multiplexed and quantitative surface proteom...
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نفت خام با بیش از340 فراورده یکی از اصلی ترین منابع انرژی و نیروی محرکه اقتصادی جهانی است و ایران 9 درصد از کل مخازن نفت جهان را در اختیار دارد. با توجه به فراوانی دفعاتی که یک تانکر یا بشکه ی نفت منتقل و ذخیره می شود، تصادف و نشت آن اجتناب ناپذیر است. احتمالاً کل میزان نفتی که از طریق فعالیّت های انسانی و یا طبیعی وارد دریا می شود می تواند سطح همه ی اقیانوس ها تا ضخامت20 مولکول بپوشاند. پاکسازی ...
Cell-free Protein Synthesis in Femtoliter Microchambers for Arraying Ultra-high Density Protein Spots
Current methodologies for arraying proteins using cell-free protein synthesis on a chip have spatial limitations that prevent reaching ultra-high density necessary for high throughput analysis. To circumvent this, we developed an on-chip method based on microcompartmentalization of protein synthesis. Proteins are synthesized in arrayed micrometer scale chambers from confined DNA template molecu...
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ژورنال
عنوان ژورنال: Microsystems & Nanoengineering
سال: 2019
ISSN: 2055-7434
DOI: 10.1038/s41378-019-0099-5